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本文通过冷冻切片琥珀酸脱氢酶(SDH)和不同梯度pH值预温育后肌球蛋白ATP酶染色,半薄切片p-次苯基-二胺(PPD)-碱性复红染色,超薄切片电镜观察,以及MAGISCAN-2A半自动图像分析仪分析,对成年鸽飞翔肌(胸大肌)及腓肠肌纤维做了组化和结构分类研究。结果表明,胸大肌经PPD染色清晰地显示出两种纤维:一是红肌纤维,直经26.8μm±6.3,含大量PPD浅染和深染的两种颗粒,电
In this paper, myosin ATPase staining was performed on frozen sections of succinate dehydrogenase (SDH) and pre-incubation at different pH values. Semi-thin sections of p-phenyl-diamine (PPD) Thin section electron microscopy, and MAGISCAN-2A semi-automatic image analyzer analysis of the adult pigeon flying muscle (pectoralis major muscle) and gastrocnemius muscle fibers were organized and structural classification studies. The results showed that pectoralis major muscle clearly showed two kinds of fibers by PPD staining: one is red muscle fibers, straight through 26.8μm ± 6.3, containing a large number of PPD lightly stained and darkly stained two kinds of particles, electricity