采用２亲本杂交的方法将Ｔｎ５－ｌｕｘＡＢ标记基因系统成功地转入了分离自小麦根圈的ＰＧＰＲ菌株荧光假单胞菌（Ｐｓｅｕｄｏｍｏｎａｓｆｌｕｏｒｅｓｃｅｎｓ）Ｘｌ６菌株中。与亲本菌株相比，携有发光酶基因ｌｕｘＡＢ的标记菌株Ｘｌ６Ｌ２生理生化特征的变化很小，而且仍保留有在小麦根圈的定殖能力。Ｔｎ５－ｌｕｘＡＢ片段在Ｘｌ６Ｌ２中的稳定性很强，在没有卡那霉素选择压力的情况下，ｌｕｘＡＢ也能稳定表达。即使在４０℃温度下，Ｘｌ６Ｌ２仍能保持发光活性 The Tn5-luxAB marker gene system was successfully transferred into the PGPR strain Pseudomonas fluorescens Xl6 strain isolated from the wheat root circle using the 2-parental crossover method. Compared with the parental strain, the change of the physiological and biochemical characteristics of the marker strain Xl6L2 carrying the luxAB gene of the light-emitting enzyme was small, and still retained the colonization ability in the root circle of wheat. The Tn5-luxAB fragment is highly stable in Xl6L2 and luxAB is stably expressed in the absence of kanamycin selection pressure. Even at a temperature of 40 ℃, Xl6L2 can still maintain luminous activity