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利用trnL intron、trnL-trnF、trnS-psbC和accD-psa I等4个叶绿体DNA片段对来自湖北省的88份梨属种质资源进行系统进化和遗传多样性分析。结果表明,4个cpDNA片段共检测到变异位点11个,其中单一突变位点6个,插入/缺失(Indel)位点5个。acc D-psa I多态性最高,其变异位点数、核苷酸多态性和单倍型多样性均为最高。供试梨种质的核苷酸多样性和单倍型多样性分别为0.00112和0.769;Tajima’s D检验值在P>0.10水平上均不显著,表明所检测的4个区域以及合并后的片段均遵循中性进化模型;4个序列合并共检测到叶绿体单倍型10个,其中兴山梨种质中检测到的单倍型最多,荆门其次;Hap2和Hap5是2个主要单倍型,分别占总样本数的31.82%和30.68%;中介邻接网络图显示东方梨和西洋梨独立进化,而较为原始的稀有单倍型Hap8和Hap9均位于荆门,暗示该地区可能为砂梨的起源中心或多样性中心之一。
The phylogeny and genetic diversity of 88 Pearlus germplasm resources from Hubei province were analyzed using four chloroplast DNA fragments, trnL intron, trnL-trnF, trnS-psbC and accD-psaI. The results showed that 11 of the 4 cpDNA fragments were detected, including 6 single mutation sites and 5 Indel sites. acc D-psa I had the highest polymorphism, with the highest number of variation sites, nucleotide polymorphisms and haplotype diversity. The nucleotide diversity and haplotype diversity of tested pear germplasm were 0.00112 and 0.769, respectively. The Tajima’s D test value was not significant at P> 0.10, indicating that all the four regions tested and the combined fragments Followed by a neutral evolution model; 10 chloroplast haplotypes were detected by 4 sequence combinations, among which the highest haplotypes were detected in germplasm of Xingshan pear, followed by Jingmen; Hap2 and Hap5 were the two major haplotypes, accounting for The average number of samples was 31.82% and 30.68%, respectively. The intermediary adjoining network showed the independent evolution of Oriental pear and Pear pear, while the more primitive rare haplotypes Hap8 and Hap9 were located in Jingmen, indicating that the region may be the origin or diversity of sand pear One of the centers