论文部分内容阅读
基于伏牛山抗癌药用植物山豆根,仙鹤草,马鞭草,绞股蓝,五倍子,猫爪草,小蓟,蛇床子,紫花地丁,重楼和半枝莲的传统和现代抗肿瘤应用,研究了这11种药物对肝癌细胞中的抑制活性。通过萃取获得药物不同浓度的乙醇提取物后,使用噻唑蓝(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT)法测定了Hep G2肝癌细胞接受不同药物刺激后的生长状况。其中,五倍子、重楼和半枝莲的乙醇提取物活性最强,对肿瘤细胞的杀伤率在70%以上。进一步的研究显示,五倍子、重楼和半枝莲的抗肿瘤作用为呈时间和浓度依赖关系。并且,其对肿瘤细胞杀伤结果是通过诱导其凋亡而实现的。此研究结果证明,五倍子、重楼和半枝莲不同体积分数的醇提取物对于肝癌Hep G2细胞有浓度依赖和时间相关的体外生长抑制和凋亡诱导作用,为其临床应用和进一步研究奠定基础。
Based on the traditional and modern antitumor applications of Funiu Mountain anticancer medicinal plants, including Fructus solanum, Agrimony, Verbena, Gynostemma, gallnut, cat’s claw grass, thistle, Cnidium, Viola yedoensis, The inhibitory activities of these 11 drugs on hepatoma cells were studied. Hep G2 hepatocarcinoma cells were treated with different drugs (MTT assay) after the different concentrations of ethanol extract were obtained by extraction. After the growth status. Among them, gallnut, re-building and Scutellaria barbata ethanol extract the strongest activity, the killing rate of tumor cells in more than 70%. Further studies have shown that anti-tumor effect of Galla chinensis, Rehmannia glutinosa and Scutellaria barbata in a time and concentration-dependent manner. And, its killing of tumor cells results by inducing apoptosis. The results of this study demonstrate that alcohol extracts of Galla chinensis, Rehmannia glutinosa and Scutellaria barbata have a concentration-dependent and time-dependent in vitro growth inhibition and apoptosis-inducing effect on Hep G2 cells, laying a foundation for their clinical application and further research .