本研究以黑木耳与毛木耳的营养缺陷型单倍体为亲本,采用原生质体电融合技术获得了8株融合子,从中选育出了具有杂种优势的冀杂3号生产菌株。此成果已于1989年3月通过鉴定。现将研究过程简介如下:材料与方法(一)菌种Aa-1(黑木耳营养缺陷型单倍体,需补充天门冬氨酸)。AP-243(毛木耳营养缺陷型单倍体,需补充酵母膏)。(二)培养基(1)完全培养基(CM):侧耳子实体浸提液,加葡萄糖2%,酵母膏0.5%,用于培养菌丝体供制 In this study, the auxotrophic haploid haploids of Auricularia auricular and Auricularia auricula were used as parents, 8 fusion strains were obtained by protoplast electrofusion technology, and the hybrid strain Jihe 3 was bred. This result was passed in March 1989 appraisal. Now the research process is as follows: Materials and methods (A) strain Aa-1 (black fungus auxotrophic haploid need to be supplemented with aspartate). AP-243 (Hairy Auricular auxotrophic haploid supplemented with yeast extract). (B) medium (1) complete medium (CM): Pleurotus ostreatus extract, plus 2% glucose, yeast extract 0.5%, used to culture mycelium for the system