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应用毛细管电泳同场放大样品电堆积和电化学检测一元胺技术,其特点是简单、快速和低成本。系统地研究了一元胺分离和在线富集的参数与传统的电动进样的关系,检测灵敏度提高5000倍,可用于人尿中未经离线样品预富集直接分析,灵敏度提高约50倍。用含有同量盐的浓度相似的人尿的标准的一元胺溶液绘制了内标校准曲线,每种一元胺的校准曲线的线性范围分别为1.0×10-9~2.5×10-8mol/L,并且这些一元胺的检测限(信噪比)都在sub-nmol/L浓度范围内(6.0×10-10mol/L)。
Applications Capillary electrophoresis with field amplification Sample electrowinning and electrochemical detection of monoamine technology, which is characterized by simple, fast and low cost. The relationship between the monoamine separation and on-line enrichment parameters and the traditional electrokinetic injection was studied systematically. The sensitivity of detection increased 5000-fold, which could be used in the direct analysis of human urine without pre-enrichment of offline samples. The sensitivity was improved about 50 times. Internal standard calibration curves were plotted against a standard solution of human urine containing similar amounts of salt, with linear calibration curves for each monoamine of 1.0 × 10-9 to 2.5 × 10-8 mol / L, respectively, The limits of detection (S / N) of these monoamines were all in the sub-nmol / L concentration range (6.0 × 10-10 mol / L).