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研究睾酮对生精功能障碍大鼠的作用,以及对CMTM家族各蛋白表达的影响。采用棉酚(50mg·kg-1)和环磷酰胺(20mg·kg-1)建立大鼠精子发生障碍模型,给予丙酸睾酮(5mg·kg-1,隔日给药,共6次)后取左侧附睾尾,收集精子。采用CASA图像分析系统对精子进行数量和活力分析,取睾丸组织采用HE染色进行形态学检查,采用蛋白印记方法(Western blotting)对CMTM家族各成员进行蛋白水平表达研究。研究发现,棉酚和环磷酰胺均可使睾丸组织发生明显的病理改变,降低精子数量和活力,而睾酮(5mg·kg-1)具有一定的改善作用。研究CMTM家族表达情况发现,棉酚和环磷酰胺均可降低雄性大鼠睾丸组织中CMTM4的表达,睾酮(5mg·kg-1)可显著提高CMTM4表达;棉酚可显著增加睾丸中CMTM2的表达,给予睾酮后CMTM2表达量显著下降,而环磷酰胺对于CMTM2影响不显著;棉酚和环磷酰胺对于CKLF1、CMTM3和CMTM5的表达影响不显著,睾丸组织中未检测到CMTM6、CMTM7和CMTM8的表达。研究结果表明,睾酮(5mg·kg-1)的生精作用可能与影响CMTM家族个别成员表达有关,趋化因子CMTM2和CMTM4可能在精子发生过程具有作用。
To study the effect of testosterone on spermatogenic dysfunction in rats and its effect on the expression of CMTM family of proteins. The rat model of spermatogenesis was established by gossypol (50 mg · kg -1) and cyclophosphamide (20 mg · kg -1), and testosterone propionate (5 mg · kg -1) Left epididymis tail, collecting sperm. The numbers and viability of sperm were analyzed by CASA image analysis system. The testicular tissues were examined by HE staining for morphological examination. Western blotting was used to study the protein expression of CMTM family members. The study found that gossypol and cyclophosphamide can make significant pathological changes in testicular tissue, reducing sperm count and vitality, and testosterone (5mg · kg-1) has some improvement. Study of CMTM family expression found that gossypol and cyclophosphamide can reduce the expression of CMTM4 in testicular tissue of male rats, testosterone (5mg · kg-1) can significantly increase the expression of CMTM4; gossypol can significantly increase the expression of CMTM2 in testis , The expression of CMTM2 decreased significantly after administration of testosterone, while cyclophosphamide had no significant effect on CMTM2; Gossypol and cyclophosphamide had no significant effect on the expression of CKLF1, CMTM3 and CMTM5, but no detectable levels of CMTM6, CMTM7 and CMTM8 expression. The results showed that the testosterone (5mg · kg-1) spermatogenesis may affect the expression of individual members of the CMTM family of chemokines CMTM2 and CMTM4 may play a role in the process of spermatogenesis.