论文部分内容阅读
本文介绍应用自制的设备所进行的毛细管色谱实验,并列举若干具体应用的实例,对毛细管色谱的定性和定量问題作一初步探讨。通过实验,我们系统地掌握了毛细管色谱柱中固定液的涂渍技术和微量、快速进样方法。用一根长82米的玻璃毛细管色谱柱,能将一国产汽油餾分,在90分钟內分离出120个色谱峯,效能达到海米2500理论塔片(正庚烷)。这一快速色谱法,从进样开始,分离了7个化合物,仅用了约0.9
This article describes the use of home-made equipment for capillary chromatography experiments, and lists some examples of specific applications, qualitative and quantitative analysis of capillary chromatography to make a preliminary study. Through experiments, we systematically grasped the technique of coating the fixed liquid in the capillary column and the method of micro-injection and rapid injection. With a 82-meter-long glass capillary column, a domestic gasoline fraction can be separated in 90 minutes, 120 peaks, the theoretical performance of plate Heme 2500 (n-heptane). With this flash chromatography, seven compounds were separated from the injection and only used about 0.9