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本文研究了汉防己甲素(简称汉甲素)对胶原蛋白的直接作用。将汉甲素与Ⅰ型胶原(1:1)在酸性条件或中性条件下共同保温24小时后,部分胶原与汉甲素结合产生沉淀。进一步用SDS-PAGE电泳法和Sepharose-6B分子筛层析法观察经汉甲素作用后上清液中胶原分子量的改变,发现Ⅰ型胶原的γ、β聚集体及α链均被解聚并分解,产生小分子肽链。证明汉甲素可以与胶原等大分子蛋白质结合,并将其分解。这可能是汉甲素减缓矽肺纤维化进程的原因之一。在小剂量汉甲素作用下(1mg汉甲素:167mgⅠ型胶原),胶原未受到明显降解。
This article studied the direct effect of tetrandrine (collagen) on collagen. After the 24-hour incubation of anacardium and type I collagen (1:1) under acidic or neutral conditions, some collagen and anacardium bound to precipitate. The SDS-PAGE electrophoresis and Sepharose-6B molecular sieve chromatography were used to observe the changes in the molecular weight of collagen in the supematants after treatment with tigrin A. It was found that the γ, β aggregates and α-chains of type I collagen were all depolymerized and decomposed. Produces small peptide chains. It is proven that anacardium can bind to macromolecular proteins such as collagen and decompose it. This may be one of the reasons for the slowing down of the fibrosis progression of silicosis. Under the effect of small doses of anacardium (1 mg anaylamine: 167 mg collagen I), collagen was not significantly degraded.