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目的:研究吡喹酮治疗血吸虫病肝纤维化小鼠前后血清一氧化氮及肝内诱导型一氧化氮合酶活性的变化。方法:制备血吸虫病肝纤维化小鼠模型,应用免疫组化染色方法和多媒体彩色病理图文分析系统定量观察吡喹酮治疗前后肝脏诱导型一氧化氮合酶含量的变化,并通过化学还原反应检测血清一氧化氮的水平变化。结果:吡喹酮治疗组肝内iNOS和血清NO的含量均低于感染组,P均小于0.01。结论:吡喹酮可显著降低血吸虫病肝纤维化小鼠肝脏诱导型一氧化氮合酶活性及血清NO含量而发挥抗血吸虫病肝纤维化的作用。
Objective: To study the changes of serum nitric oxide and inducible nitric oxide synthase in liver of schistosomiasis mice treated with praziquantel before and after treatment. Methods: The liver fibrosis model of schistosomiasis was prepared. The changes of hepatic inducible nitric oxide synthase (IOS) content before and after praziquantel treatment were quantitatively observed by immunohistochemical staining and multimedia color chart analysis system. The chemical reduction reaction Serum levels of nitric oxide were measured. Results: The contents of iNOS and serum NO in the praziquantel group were lower than those in the infected group (P <0.01). CONCLUSION: Praziquantel can significantly reduce liver hepatic inducible nitric oxide synthase activity and serum NO content in schistosomiasis mice and play a role in anti-schistosomiasis hepatic fibrosis.