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建立了反相高效液相色谱-蒸发光散射检测器(RP-HPLC-ELSD)测定紫苏子油中α-亚麻酸含量的方法。紫苏子油样用0.5m o l/L的KOH-CH3OH溶液于60℃水浴中皂化20m in、6m o l/L的盐酸酸化后用无水乙醚萃取,再用氮气流吹干乙醚,用甲醇溶解并定容,然后直接进行RP-HPLC-ELSD分析。结果表明,当α-亚麻酸质量为6.2~45.4μg时,其峰面积A的对数(lgA)与α-亚麻酸质量m(μg)的对数(lgm)呈线性关系,线性方程为lgA=1.367 5.lgm-1.628,相关系数为0.997 3(n=5)。以信噪比S/N=3计算,方法的检出限为0.11μg。被分析的紫苏子油中α-亚麻酸的含量为6.79%(质量分数),相对标准偏差(RSD)为5.1%(n=5);平均回收率为102%,RSD为6.3%(n=5)。该方法简单、快速、准确。
A reversed-phase high performance liquid chromatography coupled with evaporative light scattering detector (RP-HPLC-ELSD) was developed for the determination of α-linolenic acid in perilla oil. Perilla oil samples were saponified with hydrochloric acid of 6 mol / L in 0.5 mol / L KOH-CH3OH solution at 60 ℃ for 20 min and then extracted with anhydrous ether. The mixture was further dried with diethyl ether, dissolved in methanol Set the volume, and then directly RP-HPLC-ELSD analysis. The results showed that when the mass of α-linolenic acid was 6.2-45.4 μg, the logarithm of the peak area A (lgA) was linear with the logarithm of the mass of α-linolenic acid (μg) (lgm) and the linear equation was lgA = 1.367 5.lgm-1.628 with a correlation coefficient of 0.997 3 (n = 5). The signal-to-noise ratio S / N = 3 calculation, the detection limit of the method is 0.11μg. The content of α-linolenic acid in the perilla oil analyzed was 6.79% (mass fraction) and the relative standard deviation (RSD) was 5.1% (n = 5). The average recovery was 102% and the RSD was 6.3% (n = 5). The method is simple, fast and accurate.