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试验所用材料为前一篇文章所述的10个杨树品种的盆栽和圃地插条苗,株行距1×1米。用Arnon的方法[1]在6月,7月、9月份分别取各个品种的叶序6、9、12(从已展开叶起算第6、9、12片叶)为材料,提取叶绿素,在75Ⅰ分光光度计上测chla(叶绿素a)和chlb(叶绿素b)的含量,计算总含量及Chla/Chlb的比值。分析品种间的差异及不同生长季节的变化情况。
The materials used for the experiment were potted and nursery stock cuttings of 10 poplar varieties as described in the previous article, spaced 1 x 1 m apart. Arnon method [1] in June, July, September, respectively, the leaf sequences of each species 6,9,12 (from the leaf has been expanded count the first 6,9,12 leaves) as the material, extraction of chlorophyll in The content of chla (chlorophyll a) and chlb (chlorophyll b) were measured by 75 Ⅰ spectrophotometer to calculate the total content and the ratio of Chla / Chlb. Analysis of the differences between varieties and changes in different growing seasons.