论文部分内容阅读
目的:探讨KLOTHO基因第4外显子C-1818T多态性与中国苏皖地区汉族人群急性冠状动脉综合征(acute coronary syndrome,ACS)的可能关系。方法:应用聚合酶链反应(polymerase chain reaction,PCR)及基因芯片(gene microarray)技术对375例ACS患者(ACS组)和235例经相关检查排除冠心病(CAD)者(对照组)进行KLOTHO基因C-1818T多态性的检测。结果:与对照组相比:①ACS患者CC、CT和TT基因型以及T等位基因型频率分布无显著差异;②老年人(≥60岁)、男性和老年男性ACS患者的CT基因型频率均明显升高(P值分别为0.031、0.035和0.041)。在调整相关危险因素并经多元Logistic回归分析后,CT基因型与老年人和老年男性ACS均存在相关关系(P值分别为0.010和0.047),与男性ACS无明显相关关系(P=0.103)。结论:在中国苏皖地区汉族人群中,KLOTHO基因C-1818T多态性CT基因型与老年和老年男性ACS的发病均存在相关性。
Objective: To explore the possible relationship between C-1818T polymorphism of exon 4 of KLOTHO gene and acute coronary syndrome (ACS) in Han population in Jiangsu and Anhui Provinces. Methods: 375 patients with ACS (ACS group) and 235 patients with coronary artery disease (CAD) who underwent coronary angiography (control group) were subjected to KLOTHO by polymerase chain reaction (PCR) and gene microarray Detection of Gene C-1818T Polymorphism. Results: Compared with the control group: ① There were no significant differences in the frequency distribution of CC, CT and TT genotypes and T allele between ACS patients; ② The frequencies of CT genotypes in elderly ACS patients (≥60 years), male and elderly men (P values were 0.031, 0.035 and 0.041, respectively). There was a correlation between CT genotypes and ACS in elderly and elderly men (P = 0.010 and 0.047, respectively) after adjusted for risk factors and analyzed by multivariate logistic regression analysis. There was no significant correlation between CT genotypes and male ACS (P = 0.103). Conclusion: In Chinese Han population in Jiangsu and Anhui Provinces, the CT genotype of KLOTHO gene C-1818T polymorphism is correlated with the incidence of ACS in elderly and elderly males.