作者建立一个新的摹拟动脉壁结构的内皮和平滑肌细胞体外联合培养模型,并以此检测内皮、平滑肌细胞在合成和分泌组织型纤溶酶原激活物(t-PA)中的相互影响。结果表明,不同细胞组分的条件培养液(CM)中,t-PA活性有明显差异。内皮的CM中,t-PA活性最高,而平滑肌的CM中则最低。两种细胞联合培养时,培液的t-PA活性也明显比单纯内皮的降低。提示了细胞间的相互作用对血管壁溶栓机制的影响。 The authors established a new in vitro co-culture model of endothelial cells and smooth muscle cells, which mimicked the arterial wall structure, and examined the interaction between endothelial and smooth muscle cells in the synthesis and secretion of tissue-type plasminogen activator (t-PA). The results showed that the t-PA activity in the conditioned medium (CM) of different cell components was significantly different. Endothelium in the CM, t-PA highest activity, while the smooth muscle CM is the lowest. When the two cells were cultured in combination, the t-PA activity of the culture medium was significantly lower than that of the pure endothelium. Suggesting the effect of cell-cell interactions on the thrombolytic mechanism of the vessel wall.