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从一例诊断为高分化鳞癌病例的鼻咽部肿物的活检组织中,建立了上皮样细胞株和梭形细胞株,根据细胞的生长特性,染色体分析,异种移植和电子显微镜观察等结果,证实这两株细胞为鳞癌细胞。梭形细胞可能来源于上皮样癌细胞。细胞培养过程中出现大量圆细胞,经过一年多的培养未能获得类淋巴母细胞株。对这两株细胞进行了电子显微镜,间接免疫荧光和抗补体免疫荧光试验的检查,均未发现类疱疹病毒或其它病毒颗粒,也未发现病毒的早期抗原或核抗原(EBNA)。
From a biopsy of a nasopharyngeal mass diagnosed as a case of well-differentiated squamous cell carcinoma, epithelioid and fusiform cell lines were established, based on cell growth characteristics, chromosome analysis, xenotransplantation, and electron microscopy observations. The two cells were confirmed as squamous cell carcinoma cells. Spindle cells may be derived from epithelioid carcinoma cells. A large number of round cells appeared in the cell culture process, and lymphoblastoid cell lines could not be obtained after more than one year of culture. Electron microscopy, indirect immunofluorescence and anti-complementary immunofluorescence tests were performed on these two cells. No herpesvirus or other viral particles were found, and no early antigen or nuclear antigen (EBNA) of the virus was found.