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目的观察自由基清除剂依达拉奉对急性一氧化碳中毒性脑病大鼠空间记忆及海马CA1区神经元凋亡的影响。方法雄性SD大鼠40只,随机均分为正常组、急性一氧化碳中毒性脑病(TE-ACMP)组、生理盐水组、依达拉奉组4组。采用Morris水迷宫检测4组SD大鼠的空间记忆,以平均逃避潜伏期衡量空间记忆能力;TUNEL法检测神经元海马CA1区神经元凋亡情况。结果 ACMP组较正常组大鼠平均逃避潜伏期明显延长(P<0.05),而依达拉奉组大鼠平均逃避潜伏期较TE-ACMP组和生理盐水组均显著缩短(P<0.05)。与正常组相比,TE-ACMP组海马CA1区凋亡指数明显升高(P<0.01)。与TE-ACMP组、生理盐水组相比,依达拉奉组海马CA1区凋亡指数明显降低(P<0.01)。结论依达拉奉可改善TE-ACMP大鼠受损的空间记忆能力,抑制TE-ACMP大鼠海马CA1区神经元凋亡,可用于临床防治一氧化碳中毒性脑病。
Objective To observe the effect of free radical scavenger edaravone on the spatial memory and neuron apoptosis in hippocampal CA1 area of rats with acute carbon monoxide poisoning encephalopathy. Methods Forty male Sprague-Dawley rats were randomly divided into four groups: normal group, TE-ACMP group, normal saline group and edaravone group. Morris water maze was used to detect the spatial memory of four groups of SD rats to measure the mean memory space evasion latency. Neuronal apoptosis was detected by TUNEL in hippocampal CA1 region. Results The mean escape latency of ACMP group was significantly longer than that of normal group (P <0.05). The mean escape latency of edaravone group was significantly shorter than that of TE-ACMP group and saline group (P <0.05). Compared with the normal group, apoptosis index of hippocampal CA1 area in TE-ACMP group was significantly increased (P <0.01). Compared with TE-ACMP group and saline group, apoptosis index of hippocampal CA1 area of edaravone group was significantly lower (P <0.01). Conclusion Edaravone can improve the impaired spatial memory in TE-ACMP rats and inhibit neuronal apoptosis in hippocampal CA1 area of TE-ACMP rats. It may be used in clinical prevention and treatment of carbon monoxide poisoning encephalopathy.