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在测定单价、二价和三价金属盐和杀菌物质等对水稻白叶枯病菌[Xantho-monas oryzae (Uyeda et Ishiyama)Dowson]噬菌体影响的过程中,发现铁盐可以钝化OP1型的游离噬菌体,而时已经被吸附的噬菌体则完全没有影响。铁盐的钝化噬菌体主要是铁离子的作用。三价铁盐的钝化作用比二价亚铁盐强。对低浓度的噬菌体悬浮液(n×10~4/毫升),其最低有效浓度为3-5×10~(-3)M;对高浓度(n×10~(8-9)/毫升)的噬菌体悬浮液,其最低有效浓度为1×10~(-3)M。铁盐溶液的浓度超过1×10~(-3)M时,不仅使游离噬菌体全部钝化,对细菌也有致死作用。铁盐对噬菌体的作用,与噬菌体抗血清的作用在有些方面是相似的。在噬菌体性状的测定和有些其他研究中,可以用铁盐溶液代替抗血清。例如,在OP1型噬菌体的一步生长曲线实验中,用铁盐或抗血清处理所得的结果是非常接近的。
In determining the effect of monovalent, divalent and trivalent metal salts and bactericidal substances on the phage of Xantho-monas oryzae (Uyeda et Ishiyama) Dowson, it was found that iron salts can inactivate OP1-type free phages , While bacteriophages that have been adsorbed have no effect at all. Passivation of iron salt phage is mainly the role of iron ions. The passivation of ferric salts is stronger than that of divalent ferrous salts. For the low concentration of phage suspension (n × 10 ~ 4 / ml), the minimum effective concentration is 3-5 × 10 -3 M; for high concentration (n × 10 ~ (8-9) / ml) Of the phage suspension, the lowest effective concentration of 1 × 10 ~ (-3) M. When the concentration of iron salt solution exceeds 1 × 10 -3 M, not only the free phage is completely passivated, but also the bacterium. The effect of iron salts on phages is similar in some respects to that of phage antisera. In the determination of phage traits and in some other studies iron antisera can be used instead of antisera. For example, in the one-step growth curve experiments of OP1 phage, the results obtained with iron salts or antisera were very close.