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采用高效液相色谱法,在同一色谱条件下对稻·噁乳油中所含有效成分稻瘟灵和噁霉灵同时进行含量测定。色谱条件为:使用Nova-PaK C18250mm×4.6mm(i.d)不锈钢色谱柱,流动相v(甲醇)∶v(水)=70∶30,流速1mL/min,检测波长为220nm。测定方法中稻瘟灵和噁霉灵的回收率分别在98.8%～100.3%和98.6%～100.0%之间,变异系数分别为0.54%和0.52%,证明本方法快速、准确、可靠。
Using high performance liquid chromatography, under the same chromatographic conditions, the contents of the active ingredients of rice blast and hymexazol contained in the rice and dark-cream were simultaneously determined. The chromatographic conditions were as follows: a Nova-PaK C18 250 mm × 4.6 mm (i.d) stainless steel column was used with the mobile phase v (methanol): v (water) = 70:30, the flow rate of 1 mL / min and the detection wavelength of 220 nm. The recovery rates of rice blasting and hymexazol were 98.8% -100.3% and 98.6% -100.0%, respectively, and the coefficients of variation were 0.54% and 0.52%, respectively. The results showed that the method was rapid, accurate and reliable.