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目的:检测白介素-8受体CXCR1和CXCR2在系统性红斑狼疮(SLE)患者外周血CD14+单核细胞上的表达,探讨其与SLE疾病活动的相关性和可能涉及的SLE炎症发病机制。方法:36例活动期SLE患者和34例健康志愿者,采用流式细胞术(FCM)检测CXCR1、CXCR2在SLE患者和健康志愿者外周血CD14+单核细胞上的MFI表达。结果:CXCR2在SLE组外周血CD14+单核细胞上MFI表达(195.75±52.76)与对照组(298.82±51.86)相比明显降低(P<0.01);CXCR2在SLE患者外周血CD14+单核细胞上MFI表达下降与C3存在着正相关关系(rs=0.421,P=0.022),与dsDNA、SLEDAI存在着负相关关系(分别为rs=-0.390,P=0.032;rs=-0.463,P=0.011)。结论:SLE患者外周血CD14+单核细胞CXCR2的表达异常,提示CXCR2可能参与了SLE的发病过程。检测SLE患者外周血CD14+单核细胞的CXCR2表达水平,可能是评价SLE疾病活动性有价值的潜在的生物学标志之一。
OBJECTIVE: To detect the expression of interleukin-8 receptor CXCR1 and CXCR2 on peripheral blood CD14 + monocytes in patients with systemic lupus erythematosus (SLE) and to explore their correlation with SLE disease activity and the possible pathogenesis of SLE inflammation. Methods: Thirty-six active SLE patients and 34 healthy volunteers were enrolled in this study. The expression of CXCR1 and CXCR2 on CD14 + monocytes in peripheral blood of SLE patients and healthy volunteers were detected by flow cytometry (FCM). Results: The MFI of CXCR2 on peripheral blood CD14 + monocytes in SLE group (195.75 ± 52.76) was significantly lower than that in control group (298.82 ± 51.86) (P <0.01) There was a positive correlation between the decreased expression and C3 (rs = 0.421, P = 0.022), but negatively correlated with dsDNA and SLEDAI (rs = -0.390, P = 0.032; rs = -0.463, P = 0.011). Conclusion: The abnormal expression of CXCR2 in peripheral blood CD14 + monocytes in patients with SLE suggests that CXCR2 may be involved in the pathogenesis of SLE. Detecting CXCR2 expression in peripheral blood CD14 + monocytes in patients with SLE may be one of the potential biological markers for evaluating the activity of SLE disease.