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目的:观察复方绞芪方对高脂高糖饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠脂质代谢变化情况的影响,探讨其防治NASH的作用机制。方法:高脂高糖饮食喂养12周诱导SD大鼠建立NASH模型,同时以不同剂量的复方绞芪方治疗,检测大鼠血清TG、CHOL、HDL-C、LDL-C、FFA、ALT、AST和肝组织匀浆TG、CHOL含量变化,常规HE染色观察肝组织的病理改变并计算NAFLD活动度积分(NAS)。结果:模型组大鼠的血清ALT、AST、LDL-C、FFA水平以及肝组织中的CHOL、TG含量较正常组明显增加;而血清HDL-C水平明显下降;NAS计分明显升高。应用复方绞芪方进行干预后,大鼠NAS计分较模型组大鼠显著降低,肝功能明显改善,血清CHOL、TG、LDL-C、FFA水平明显下降同时HDL-C明显升高,肝组织中CHOL和TG含量也明显下降。结论:高脂高糖饮食诱导的NASH大鼠存在脂质代谢紊乱,复方绞芪方能一定程度纠正肝组织脂质代谢紊乱,这可能是其防治NASH的重要机制。
Objective: To observe the effects of compound strangulation on the changes of lipid metabolism in rats with non-alcoholic steatohepatitis (NASH) induced by high-fat and high-sucrose diet and to explore its mechanism of action in preventing and treating NASH. Methods: NASH rats were induced by high fat and high sugar diet for 12 weeks. At the same time, the rats were treated with different doses of compound stilbene. Serum levels of TG, CHOL, HDL-C, LDL-C, FFA, ALT, AST And liver homogenates TG, CHOL content changes, routine HE staining observed pathological changes of liver tissue and calculate NAFLD activity score (NAS). Results: Serum levels of ALT, AST, LDL-C, FFA and CHOL, TG in liver tissue of rats in the model group were significantly higher than those in the normal group, while serum HDL-C levels were significantly decreased; NAS score was significantly increased. Compared with the model group, the scores of NAS decreased significantly and the liver function improved significantly. The levels of serum CHOL, TG, LDL-C and FFA were significantly decreased while HDL-C was significantly increased CHOL and TG content also dropped significantly. CONCLUSION: The lipid metabolic disorder is induced in NASH rats induced by high fat and high glucose diet. Compound Radix Shuangqi can correct the disorder of lipid metabolism in liver tissue to a certain extent, which may be an important mechanism of prevention and treatment of NASH.