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目的体外诱导骨髓间充质干细胞(mesenchymal stem cells,MSCs)分化为胰岛样细胞,用于干预治疗糖尿病大鼠。方法取周龄大鼠骨髓细胞,进行MSC培养纯化扩增,用bFGF、胰岛素等培养体系诱导MSC向胰岛细胞转化;双硫腙染色鉴定胰岛样细胞。使用链脲菌素(Streptozotocin,STZ)制作糖尿病大鼠模型,将诱导分化的胰岛样细胞移植至糖尿病模型大鼠,观察干预治疗效果。结果体外诱导MSCs由长梭形变成多角形并逐渐聚集成圆形,双硫腙染色后细胞团成亮红色,表明为胰岛样细胞。糖尿病模型大鼠移植诱导分化胰岛样细胞1周后,多饮、多尿症状减轻,10d后,血糖值由(21.352±3.150)mmol/L降低为(14.604±3.236)mmol/L,与对照组(22.256±4.051)mmol/L比较,差异无统计学意义(P>0.05);尿糖值由(18.00±1.00)g/L降低为(8.50±0.25)g/L,与对照组(20.00±0.18)g/L比较,差异有统计学意义(P<0.05)。结论骨髓间充质干细胞可在体外诱导分化为胰岛样细胞;骨髓间充质干细胞诱导分化的胰岛样细胞可以降低糖尿病大鼠血糖、尿糖值水平,可能具有治疗糖尿病作用。
Objective To induce the differentiation of mesenchymal stem cells (MSCs) into islet-like cells in vitro for the interventional treatment of diabetic rats. Methods Bone marrow cells from week-old rats were harvested and purified by MSC culture. MSCs were transformed into pancreatic islet cells by culture system of bFGF and insulin, and islet-like cells were identified by dithizone staining. Diabetic rat model was made by using streptozotocin (STZ), and the differentiated islet-like cells were transplanted to diabetic rats to observe the intervention effect. Results MSCs induced by long-spindle transform into polygons and gradually gathered into round shape. After dithizone staining, MSCs became bright red, indicating islet-like cells. After 1 week, the symptoms of polydipsia and polyuria in diabetic model rats were induced to differentiate into islet cells. After 10 days, the blood glucose level decreased from (21.352 ± 3.150) mmol / L to (14.604 ± 3.236) mmol / L, (22.256 ± 4.051) mmol / L, the difference was not statistically significant (P> 0.05); the urine glucose value decreased from (18.00 ± 1.00) g / L to (8.50 ± 0.25) g / 0.18) g / L, the difference was statistically significant (P <0.05). Conclusion Bone marrow mesenchymal stem cells can differentiate into islet-like cells in vitro. Bone marrow-derived mesenchymal stem cells differentiate islet-like cells can reduce the level of blood glucose and urine sugar in diabetic rats, which may be useful in the treatment of diabetes.