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Dear Editor,As an essential element for pollen tube tip growth,actin cytoskeleton has been a focus for decades.Recently,both rapid-freeze and room-temperature f
【机 构】
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Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, and College of
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Dear Editor,As an essential element for pollen tube tip growth,actin cytoskeleton has been a focus for decades.Recently,both rapid-freeze and room-temperature fixation have revealed the presence of a subapical cortical F-actin (actin fringe)as a consistent feature of lily pollen tubes (Lovy-Wheeler et al.,2005).The actin fringe consists of a palisade of short longitudinally oriented actin cables that start about 1-5μm behind the extreme apex and extend basally for an additional 5-10 μm (Lovy-Wheeler et al.,2005).Meanwhile,observations in live cells using Lifeact-mEGFP,an actin probe consisting of the first 17 amino acids from the budding yeast ABP-140 fused to GFP,which does not affect the dynamics of actin,compellingly support the presence of a cortical actin fringe in lily pollen tubes (Vidali et al.,2009).
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