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【目的】比较广东、广西产两面针不同部位中氯化两面针碱的含量差异。【方法】采用体积分数70%甲醇超声提取,高效液相色谱法(HPLC)测定,色谱柱为Hypersil ODS C18(4.6mm×200mm,5μm);以乙腈为流动相A,1.22g/L甲酸—三乙胺(pH4.5)为流动相B,梯度洗脱。【结果】氯化两面针碱的平均回收率和sR分别为98.32%、1.85%。广西、广东产两面针根中均含有氯化两面针碱,且含量最高;广西产两面针茎中含氯化两面针碱,而广东产茎中含或不含氯化两面针碱,其含量低于广西产,两产地叶中均不含氯化两面针碱。【结论】不同来源和不同部位两面针中氯化两面针碱含量存在一定的差异,该测定方法简单、快速、准确可靠,可为两面针药用部位的选取和资源合理开发利用提供参考。
【Objective】 To compare the content of nitidine chloride in different parts of Guangdong and Guangxi. 【Method】 Hypersil ODS C18 (4.6 mm × 200 mm, 5 μm) was used for the determination by HPLC with 70% methanol by volume fraction. The mobile phase A was acetonitrile, the mobile phase A was 1.22 g / L formic acid- Triethylamine (pH 4.5) as mobile phase B, gradient elution. 【Result】 The average recovery and sR of nitidine chloride were 98.32% and 1.85% respectively. Guangxi, Guangdong produced two-sided needle root contains nitidine chloride, and the highest content; Guangxi produced on both sides of the needles containing nitidine chloride, and Guangdong stem with or without nitidine chloride, its content Lower than the Guangxi production, leaves of the two producing areas are not chlorinated nitidine chloride. 【Conclusion】 There are some differences in the content of alkali of Nitidine lilium between different sources and different parts. The method is simple, rapid, accurate and reliable, which can provide references for the selection of medicinal parts and the rational development and utilization of resources.