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目的研究舒芬太尼2种镇痛方法的效果及对家兔免疫球蛋白的影响。方法健康家兔18只,随机分为3组,每组6只。各组家兔均予足底注射2%甲醛溶液0.5mL致痛,A、B组致痛后分别经静脉和硬膜外注射舒芬太尼0.66和0.92μg·kg-1,C组静脉注射等容量生理盐水作为对照。根据行为判断标准评估动物的疼痛反应记录评分,并检测致痛前(T0),致痛后1h(T1)、24h(T2)、48h(T3)和72h(T4)时的家兔血清中IgG、IgM和IgA水平。结果3组免疫球蛋白水平在致痛前无差异。致痛后45min内,A、B组疼痛评分稳定下降,10min后无明显疼痛,C组家兔疼痛持续较长时间。与T0时比较,3组家兔免疫球蛋白水平在T1和T2时均明显下降(P<0.01)。A组在T4时免疫球蛋白上升到正常水平,B组在T3时上升到正常水平,C组在T4时仍低于正常水平。结论舒芬太尼镇痛有助于疼痛与应激引起的家兔体液免疫功能抑制的稳定和恢复,硬膜外镇痛途径疗效优于静脉镇痛。
Objective To study the effect of sufentanil on two kinds of analgesia and its effect on rabbit immunoglobulin. Methods Eighteen healthy rabbits were randomly divided into 3 groups with 6 rats in each group. The rabbits in each group were given 0.5mL 2% formalin solution to the plantar. The pain in groups A and B were intravenously and epidurally injected with sufentanil 0.66 and 0.92μg · kg-1, respectively. The rabbits in group C were injected intravenously Equal volume of saline as a control. The pain scores of the animals were evaluated according to behavioral criteria and the serum levels of IgG in rabbits before induced pain (T0), 1 h (T1), 24 h (T2), 48 h (T3) and 72 h , IgM and IgA levels. Results There was no difference in immunoglobulin levels between the three groups before induced pain. Within 45 minutes after the pain was induced, the pain scores of Group A and B decreased steadily, and no pain was found after 10 minutes. Pain in Group C continued for a long time. Compared with T0, immunoglobulin levels in three groups of rabbits decreased significantly at T1 and T2 (P <0.01). In group A, immunoglobulin increased to normal level at T4, group B increased to normal level at T3, and group C remained below normal level at T4. Conclusions Sufentanil is effective in stabilizing and restoring the suppression of humoral immune function induced by pain and stress in rabbits. Epidural analgesia is more effective than intravenous analgesia.