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背景:扶芳藤和银杏叶都有扩张血管等多种药理活性作用,对脑缺血急性损伤期脑组织中自由基是否有抑制作用?而这种作用将为神经保护药的研究提供依据。目的:研究扶芳藤和银杏叶对大鼠脑缺血再灌注损伤的抗氧化作用。设计:完全随机设计,对照实验研究。地点和材料:广西中医学院实验动物中心提供Wistar雄性大鼠,体质量200～250g,广西中医学院制药厂提供干燥扶芳藤茎叶和银杏叶,2001-01/08在广西中医学院进行实验。干预:实验设计者为第四、二作者。大鼠随机分为4组,第1、2组均不给药。第3组按扶芳藤40g/(kg·d),第4组按扶芳藤40g/(kg·d)和银杏叶30g/(kg·d),分别将药材水煎2次、合并药液浓缩至2×103g/L,拌入颗粒饲料喂食10d。各组大鼠均自由饮水进食。麻醉大鼠,定位后切口暴露动脉,电凝阻断椎动脉;结扎颈总动脉,15min后松开颈总动脉,造成脑完全缺血再灌注损伤模型;3h后断头取脑备用。假手术组只切开暴露,不阻断,不结扎。主要观察指标:制备脑组织匀浆,用邻苯三酚法测定超氧化物歧化酶(superoxidedismutase,SOD)的活性,用硫代巴比妥酸(2-Thiobarbituricacid)法测定丙二醛的含量。结果:与对照组比较,喂食扶芳藤的大鼠SOD活力提高22.4%,丙二醛含量降低47.9%,喂食扶芳藤和银杏叶的大鼠SOD活力提高26.4%,丙二醛含?
BACKGROUND: Both Euonymus fortunei and Ginkgo biloba have a variety of pharmacological activities such as dilation of blood vessels, and can inhibit the free radicals in brain tissue during acute cerebral ischemia. This effect will provide a basis for the study of neuroprotective drugs. Objective: To study the anti-oxidation effects of Euonymus fortunei and Ginkgo biloba on cerebral ischemia-reperfusion injury in rats. Design: Completely random design, controlled experimental study. Location and Materials: The Experimental Animal Center of Guangxi College of Traditional Chinese Medicine provided Wistar male rats with a body weight of 200-250 g. The Guangxi Traditional Chinese Medicine Institute provided dry eucommia ulmoides leaves and ginkgo biloba leaves. The experiment was conducted at Guangxi Medical College from January 2001 to August 08. Intervention: The experimental designers were the fourth and second authors. The rats were randomly divided into 4 groups, and none of the 1st and 2nd groups were administered. Group 3 was treated with Euonymus fortunei 40g/(kg·d), Group 4 was treated with Euonymus fortunei 40g/(kg·d) and Ginkgo biloba 30g/(kg·d), and the medicinal materials were decocted twice and combined together. The liquid was concentrated to 2 x 103 g/L and the pelleted feed was fed for 10 days. All groups of rats were free to drink water. Anesthetize rats, locate the incision after exposure of the artery, electrocoagulation blocking the vertebral artery; ligation of the common carotid artery, 15min after the release of the common carotid artery, causing a complete cerebral ischemia reperfusion injury model; 3h after decapitation take brain back. The sham-operated group only cut exposed, did not block, and did not ligation. MAIN OUTCOME MEASURES: Brain tissue homogenates were prepared, the activity of superoxide dismutase (SOD) was measured by pyrogallol method, and the content of malondialdehyde was measured by thiobarbituric acid (2-Thiobarbituric acid) method. Results: Compared with the control group, the SOD activity of the rats fed with Euonymus fortunei increased by 22.4%, the content of MDA decreased by 47.9%, and the SOD activity of rats fed with Euonymus fortunei and Ginkgo biloba increased by 26.4%.