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对麻疯树成熟胚乳进行组织培养获得胚乳再生植株,并对其气孔进行分析.麻疯树成熟胚乳在25℃、12 h光照条件下培养7 d愈伤组织诱导完成,2,4-D浓度为2.0 mg L-1的MS培养基愈伤诱导效果最好,诱导率达89.29%.愈伤组织在含BAP的改良培养基上培养至黄绿色后转入分化培养基,在含IAA 0.25 mg L-1和ZT 1.5 mg L-1的WPM培养基上不定芽分化率达32.50%.将分化的不定芽从愈伤组织上剥离后转入含IBA、BAP和GA3的培养基上进行芽伸长培养.取胚乳不定芽叶片接种在含IBA 0.1 mg L-1、BAP 0.5 mg L-1和TDZ 0.5 mg L-1的MS培养基上诱导生芽后,再转入含IAA 0.25mgL-1、KT 0.5mg L-1、BAP 1.0 mg L-1和GA3 0.25 mg L-1的培养基上进行丛生芽的诱导,成芽率为85.2%.这些芽在含0.1 mgL-1 IBA的1/2 MS培养基上生根,大约有37.5%的芽生了根,平均有5.2条根系形成.与母本植株相比,再生的胚乳植株保卫细胞更大,且气孔密度减小.图2表6参24
The endosperm regenerated plants were obtained from mature endosperm of Jatropha curcas and the stomata were analyzed.The mature endosperm of Jatropha curcas was cultured for 7 days under the light conditions of 25 ℃ and 12 hours.The induction of 2,4-D concentration The callus induction effect of MS medium supplemented with 2.0 mg L-1 was the best, with the induction rate of 89.29% .The callus was cultivated on the modified medium containing BAP to yellow-green and then transferred to differentiation medium, The differentiation rate of adventitious buds on WPM medium with L-1 and ZT 1.5 mg L-1 reached 32.50% .Differentiated adventitious buds were detached from callus and transferred to medium containing IBA, BAP and GA3 for budding Inoculated with endosperm adventitious bud leaves were inoculated on MS medium containing 0.1 mg L-1 IBA, 0.5 mg L-1 BAP and 0.5 mg L-1 TDZ to induce germination, then transferred to IAA 0.25 mg L-1 , KT 0.5 mg L-1, BAP 1.0 mg L-1 and GA3 0.25 mg L-1, the rate of germination was 85.2% .These buds were cultured in medium with 0.1 mg L-1 IBA in 1 / 2 MS medium, about 37.5% of the buds rooted, with an average of 5.2 root formation.Compared with the female parent plants, the regenerated endosperm plants had more guard cells and reduced stomatal density. twenty four