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以苹果、姜、蒜为试材,采用对峙培养法研究了健康苹果果实、姜、蒜内的可培养内生细菌和健康苹果植株根际土壤微生物对苹果轮纹病的拮抗作用。结果表明:根际土壤中分离到可培养细菌9种,姜、蒜、苹果内分别分离到可培养菌株5种、3种和1种;对分离到的微生物进行拮抗性测定,除土2对苹果轮纹病菌菌丝生长无抑制效果外,其余菌株都对苹果轮纹病菌有抑菌效果,且从根际土壤中分离到的微生物对苹果轮纹病菌具有拮抗性的所占比例较大,为44.4%;其次为姜和蒜,所占比例分别为27.8%和16.7%。所有菌株中除蒜12,土1和土5与对照比较抑菌效果差异不显著外,其余菌株抑菌效果差异都显著。所有菌株中抑制效果最好的为姜4,其真菌菌落直径只有(30.78±0.49)mm,其次为土4,真菌菌落直径为(35.12±0.13)mm。
Apple, ginger and garlic were used as experimental materials to study the antagonism of rhizosphere microbes in healthy apple, ginger and garlic cultivars and rhizosphere soil of healthy apple plants against apple ring disease by confrontation culture method. The results showed that 9 kinds of bacteria could be isolated from rhizosphere soil, 5, 3 and 1 kinds of cultivable strains were isolated from ginger, garlic and apple respectively. The isolated microorganisms were tested for antagonism, All the isolates had antibacterial activity against the pathogen of apple rot pathogen, and the microorganisms isolated from rhizosphere soil had a larger proportion of antagonistic to apple rotten pathogen, Was 44.4%, followed by ginger and garlic, accounting for 27.8% and 16.7% respectively. All strains except garlic 12, soil 1 and soil 5 compared with the control antibacterial effect was no significant difference, the other strains were significant differences in antibacterial effect. The best inhibitory effect of all the strains was ginger 4. The fungal colony diameter was only (30.78 ± 0.49) mm, followed by soil 4, the fungal colony diameter was (35.12 ± 0.13) mm.