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目的 探讨珊瑚羟基磷灰石（CHAP）体外复合经扩增的骨髓基质细胞（BMSc）在大动物大段骨缺损中的修复能力及放射性核素骨显像技术在此过程中的应用价值。方法 中国青山羊6只分为实验组和对照组,每组3只。分别造成左侧胫骨2cm骨缺损,实验组缺损区植入CHAP和BMSc复合体,对照组不植入任何填充物。术后2、4、8周分别通过放射性核素骨显像进行监测。结果 放射性核素骨显像中感兴趣区（ROI）计数和摄取比值显示对照组在3个时间点均未见再血管化的表现及明显的成骨活动,而实验组则随着时间的延长再血管化的数量和成骨的质量呈现出上升的趋势。结论CHAP和BMSc复合体具有良好的修复大动物大段骨缺损的能力,放射性核素骨显像在修复过程中有非常准确的预测效果。
OBJECTIVE: To investigate the repair ability of Co-hydroxyapatite (CHAP) composite expanded bone marrow stromal cells (BMSc) in large segmental defects of large animals and the value of radionuclide bone imaging in this process. Methods Six Chinese goats were divided into experimental group and control group, with 3 in each group. Respectively, resulting in 2cm bone defect of the left tibia, the experimental group implanted in the defect area CHAP and BMSc complex, the control group did not implant any filler. At 2, 4, and 8 weeks after surgery, radionuclide bone imaging was performed respectively. Results The ROI count and uptake ratio in radionuclide bone scintigraphy showed that the control group showed no revascularization and significant osteogenic activity at all three time points. However, in the experimental group, The number of revascularization and the quality of osteogenesis showed an upward trend. Conclusion The CHAP and BMSc complexes have a good ability to repair large segmental defects in large animals. Radionuclide bone imaging has a very accurate prediction effect in the repair process.