论文部分内容阅读
目的:探讨白细胞介素-1β(IL-1β)及白细胞介素-13(IL-13)在溃疡性结肠炎(UC)中的作用及清肠栓对其的影响,进一步研究清肠栓治疗UC的作用机理。方法:SD大鼠56只随机分为正常组、模型组(空白组)、西药组(SASP组)、中药组(清肠栓组)共4组,每组14只。用TNBs100mg.kg-1灌肠建立大鼠溃疡性结肠炎模型,第3天开始,除正常组外,各组分别给予生理盐水、柳氮磺胺吡啶、清肠栓处理,至7天后处死动物。腹主动脉取血制备血清,采用ELISA法测定各组血清IL-1β、IL-13的含量。结果:UC大鼠血清IL-1β含量显著升高,西药组及中药组均能降低其含量。中药组与西药组相比,P<0.01。而UC大鼠血清IL-13含量显著下降,西药组及中药组均能升高其含量,P<0.01。结论:中药清肠栓可以通过降低血清IL-1β含量,上调血清IL-13含量。从而调节肠道免疫平衡,达到缓解UC的治疗目的。
Objective: To investigate the effects of interleukin-1β (IL-1β) and interleukin-13 (IL-13) in ulcerative colitis (UC) and the effect of clear bowel plug on it, and further study the treatment of clear bowel suppository The mechanism of action of UC. METHODS: Fifty-six SD rats were randomly divided into four groups: normal group, model group (blank group), western medicine group (SASP group), and traditional Chinese medicine group (Qingchang Shuan group). There were 14 rats in each group. The rat ulcerative colitis model was established with TNBs 100 mg.kg-1 enema. On the third day, except normal group, each group was treated with physiological saline, sulfasalazine, and clear bowel suppository. Animals were sacrificed 7 days later. Serum from the abdominal aorta was used to prepare serum. The levels of IL-1β and IL-13 in serum were determined by ELISA. Results: The serum IL-1β content in UC rats was significantly increased. Western medicine group and Chinese medicine group were able to reduce the content of IL-1β. Compared with the western medicine group, the Chinese medicine group P<0.01. The serum IL-13 level in UC rats was significantly decreased, and both western medicine group and Chinese medicine group were able to increase their levels, P<0.01. Conclusion: Qingchang suppository can reduce the content of IL-1β in serum and up-regulate the serum IL-13 level. In order to adjust the immune balance of the intestine, to achieve the purpose of relieving UC.