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目的:观察大黄水提物对内毒素血症模型大鼠体内血清中超氧化物歧化酶(SOD),过氧化氢酶(CAT)活力及丙二醛(MDA),过氧化氢(H2O2)含量的影响,初步探讨大黄水提物对内毒素血症模型大鼠氧化应激的保护作用。方法:将50只SD大鼠随机分为正常组,模型组,地塞米松阳性药组(7.5×10-4g·kg-1),大黄高、低剂量组(3.0,1.5 g·kg-1)。每天早晚各给药1次,连续给药7次,末次给药0.5 h后尾静脉注射内毒素(LPS)(5 mg·kg-1)复制模型,然后每间隔0.5 h测定一次动物肛温,于造模后4 h麻醉动物,剖取脾脏、胸腺、肾上腺等组织称取各脏器质量计算其脏器系数,股动脉取血制备血清,采用比色法试剂盒测定血清中SOD,CAT活力及MDA,H2O2的含量。结果:与模型组比较,大黄水提物1.5 g·kg-1能显著降低模型大鼠的体温变化指数(TRI4)(P<0.05),明显降低血清中MDA,H2O2的含量(P<0.05),显著升高SOD,CAT的活力(P<0.05)。结论:氧化应激是LPS诱发内毒素血症的重要环节,大黄可以通过上调SOD及CAT的活性,清除体内过度累积的自由基,干预模型大鼠体内氧化应激损伤的信号传导,起到一定的保护作用。
OBJECTIVE: To observe the effects of rhubarb water extract on the levels of superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and hydrogen peroxide (H2O2) in serum of rats with endotoxemia Effect of rhubarb aqueous extract on endotoxemia model of oxidative stress in rats. Methods: 50 SD rats were randomly divided into normal group, model group, dexamethasone positive group (7.5 × 10-4g · kg-1), rhubarb high and low dose group (3.0,1.5 g · kg-1 ). Each administration was given once a day in morning and evening for 7 consecutive times. After the last administration 0.5 h, LPS (5 mg · kg -1) was injected into the caudal vein to replicate the model, and then the animal’s rectal temperature was measured every 0.5 h. The animals were anesthetized 4 hours after the model was established. The spleen, thymus and adrenal gland were dissected. The organ weights were weighed to calculate the organ coefficients. The femoral artery blood was used to prepare the serum. The activities of SOD and CAT in the serum were measured by colorimetric kit And MDA, H2O2 content. Results: Compared with the model group, the water extract of rhubarb (1.5 g · kg-1) significantly reduced the body temperature index (TRI4) and the content of MDA and H2O2 in serum (P <0.05) , Significantly increased the activities of SOD and CAT (P <0.05). Conclusion: Oxidative stress is an important part of LPS-induced endotoxemia. Rhubarb can up-regulate the activity of SOD and CAT, eliminate excessive accumulation of free radicals in vivo, and interfere with the signal transduction of oxidative stress injury in model rats, The protective effect.