本文报告用间断流动离心血细胞分离法(简称 IF-CL)收集中性粒细胞治疗18倒革兰氏阴性败血症。患者年龄自4至71岁,其中急性粒细胞白血病10例,急性淋巴细胞白血病3例,慢性淋巴细胞白血病,毛细胞白血病,再生障碍性贫血,创伤和肉瘤各1例。各例患者均有严重的中性粒细胞减少(<500/μ1)以及经培养证实的革兰氏阴性败血症。所有患者均对联合抗菌素治疗至少48小时无反应,全都每天接受粒细胞输注。持续发热再加上血培养持续阳性或病情进 This article reports the use of intermittent flow cytometry (IF-CL) to collect neutrophils to treat 18 down Gram-negative sepsis. Patients ranged in age from 4 to 71 years, with 10 cases of acute myeloid leukemia, 3 cases of acute lymphocytic leukemia, 1 case of chronic lymphocytic leukemia, hairy cell leukemia, aplastic anemia, trauma and sarcoma. Each patient had severe neutropenia (<500 / μ1) as well as culture-proven Gram-negative sepsis. All patients had no response to combined antibiotic therapy for at least 48 hours and all received daily granulocyte transfusions. Continuous fever plus blood culture continued to be positive or into the disease