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本文报道一种血液淋巴细胞核内DNA主链断裂相对测定法的应用及其有关原理。基本过程是用碱性液对载片上的细胞核DNA进行解旋,然后用吖啶橙染色,用显微荧光分光光度法测定核内DNA主链断裂的相对量。本法原则上也适用于其他真核细胞。由于所用的材料极省,每次实验每组只需测定10～30个细胞,故对老年医学临床研究相当有利。在应用中我们对前人的方法作了部分修改。
This paper reports the application of a method for the relative determination of DNA backbone breaks in blood lymphocytes and its related principles. The basic procedure is to use alkaline solution to unwind the nuclear DNA on slides and then stained with acridine orange to determine the relative amount of DNA backbone breaks in the nucleus by micro-fluorescence spectrophotometry. In principle, this Law also applies to other eukaryotic cells. Due to the minimal material used, only 10 to 30 cells per group are required for each experiment, so clinical research in geriatrics is quite beneficial. In the application of our predecessors made some changes to the method.