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目的 比较研究哌拉西林、舒巴坦、哌拉西林与舒巴坦不同比例联合及阿莫西林 /舒巴坦 (2∶1) ,对临床分离 3 0 9株需氧菌和 3 0株厌氧菌的体外抗菌活性及其影响因素 (研究哌拉西林 /舒巴坦 (2∶1)对金葡球菌、大肠埃希氏菌和铜绿假单胞菌的的杀菌曲线 )。方法 采用琼脂平板稀释法及肉汤稀释法测定MIC值 ,Nitrocefin纸片法测定细菌产生的 β 内酰胺酶 ,时间杀菌曲线采用肉汤 10倍稀释法。结果 对临床分离的3 3 9株需氧菌及厌氧菌体外抗菌活性研究结果显示 ,哌拉西林及其与舒巴坦联合时 ,对革兰氏阳性球菌及厌氧菌的体外抗菌活性MIC50 为 1～ 4μg/ml,MIC90 多数低于 12 8μg/ml,优于革兰氏阴性杆菌 ,其MIC50 为 1～12 8μg/ml ,MIC90 多数高于 12 8μg/ml;单用舒巴坦对各种细菌的体外抗菌活性极差 ,多数菌的MIC50 及MIC90 分别为 64及 2 5 6μg/ml。对革兰氏阴性杆菌及厌氧菌 ,哌拉西林与舒巴坦联合时体外抗菌活性明显优于单用哌拉西林 ;对于革兰氏阳性球菌 ,哌拉西林联合舒巴坦后体外抗菌活性改善不如革兰氏阴性杆菌及厌氧菌。哌拉西林对产酶株的MIC高于非产酶株 ,联合舒巴坦后 ,产酶株MIC降低而非产酶株MIC变化不明显。不同比例联合的体外抗菌活性比较发现 ,以哌拉西林 /舒巴坦 2∶1联合时效果较好。
OBJECTIVE To compare the different combinations of piperacillin, sulbactam, piperacillin and sulbactam and amoxicillin / sulbactam (2: 1) for the clinical isolation of 309 strains of aerobic bacteria and 30 strains In Vitro Antibacterial Activity of Oxygen Bacteria and Its Influencing Factors (Study on Bactericidal Activity of Piperacillin / Sulbactam (2: 1) against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa). Methods The MIC value was determined by agar plate dilution method and broth dilution method. The Nitrocefin paper method was used to determine the β-lactamase produced by bacteria. The bactericidal curve of time was 10-fold diluted with broth. Results The in vitro antibacterial activity of 3 3 9 aerobic and anaerobic bacteria isolated from clinical isolates showed that in vitro antibacterial activity of piperacillin and its combination with sulbactam against Gram-positive cocci and anaerobic bacteria MIC50 was 1 ~ 4μg / ml, MIC90 was mostly lower than 12 8μg / ml, better than gram-negative bacilli, its MIC50 was 1 ~ 128μg / ml, MIC90 most higher than 12 8μg / ml; In vitro antibacterial activity of various bacteria is very poor, most of the MIC50 and MIC90 were 64 and 256μg / ml. In vitro antibacterial activity against Gram-negative bacilli and anaerobes, piperacillin and sulbactam was significantly better than piperacillin alone. In vitro antibacterial activity against Gram-positive cocci and piperacillin combined with sulbactam Better than Gram-negative bacilli and anaerobic bacteria. The MIC of piperacillin-producing strains was higher than that of non-producing strains and combined with sulbactam. The MIC of piperacillin-producing strains decreased but that of non-producing strains of MICs was not obvious. In vitro antibacterial activity in different proportions was found to be better with piperacillin / sulbactam 2: 1 combination.