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目的:探讨了糖基化终末产物(advanced glycation end products,AGE)及其受体(receptor for advanced glycation end products,RAGE)在糖尿病大鼠胃组织中的分布.方法:糖尿病模型组与正常组大鼠饲养8w k,测量空腹血糖、糖化血清蛋白、胃壁组织学,免疫组织化学检测AGE与RAGE在胃壁组织的表达.结果:模型组大鼠胃黏膜层(781.47μm±137.82μm vs 709.85μm±169.41μm)和黏膜下层(233.39μm±134.05μm vs109.32μm±44.43μm)的厚度较正常组显著增加(P<0.05);AGE与RAGE在模型组大鼠胃组织的黏膜层(5.66±1.90 vs 2.25±0.52,2.79±0.54 vs 1.70±0.30)和肌层(37.37±7.38 vs 24.32±4.02,4.26±0.80vs 3.59±0.37)的分布较正常组显著增加(P<0.05).结论:AGE与RAGE在糖尿病大鼠的胃组织中表达上调,该异常分布可能与糖尿病胃肠功能障碍有关.
Objective: To investigate the distribution of advanced glycation end products (AGE) and receptor for advanced glycation end products (RAGE) in the stomach of diabetic rats.Methods: The diabetic model group and the normal group The rats were fed for 8 weeks, and the fasting blood glucose, glycosylated serum protein and gastric wall histology were measured, and the expressions of AGE and RAGE in gastric tissue were detected by immunohistochemistry.Results: The gastric mucosa of model group (781.47μm ± 137.82μm vs 709.85μm ± 169.41μm) and submucosa (233.39μm ± 134.05μm vs109.32μm ± 44.43μm) were significantly increased compared with the normal group (P <0.05). AGE and RAGE in the gastric mucosa of model group (5.66 ± 1.90 vs 2.25 ± 0.52, 2.79 ± 0.54 vs 1.70 ± 0.30 and 37.37 ± 7.38 vs 24.32 ± 4.02, 4.26 ± 0.80 vs 3.59 ± 0.37, respectively) (P <0.05) .Conclusion: AGE and RAGE It is upregulated in the stomach of diabetic rats, which may be related to the gastrointestinal dysfunction of diabetes.