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内源性大麻素类物质如anandamide被认为对怀孕和胚胎发育有不良影响,在初步试验中已经验证anandamide水解酶的活性与防止子宫内anandamide水平过高及妊娠失败或女性不孕相关。本文旨在探讨是否能用该降解酶的活性改变来预测孕妇的流产情况。 方法 用特异的放射色谱法评估50例妊娠6～11周的健康孕妇外周淋巴中水解酶的活性。通过特异多克隆抗体的ELISA测定蛋白水平下的酶的表达。在进一步的研究中,测
Endocannabinoids such as anandamide, considered to have adverse effects on pregnancy and embryonic development, have been shown in preliminary tests to be associated with the prevention of anandamide hydrolase hypersecretion in pregnancy and pregnancy failure or female infertility. This article aims to explore whether the activity of the degradative enzyme can be used to predict miscarriage in pregnant women. Methods Peripheral lymphatic hydrolases were assayed in 50 healthy pregnant women with 6-11 weeks of gestation by specific radio-chromatography. The expression of the enzyme at the protein level was determined by ELISA of specific polyclonal antibodies. In further research, measure