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为了探讨在内毒素作用下的乳鼠心肌细胞 (neonatalratcardiomyocytes,NRCMs)血红素加氧酶 1(hemeoxyge nase 1,HO 1)基因的表达及其在细胞损伤中的作用 ,分别用 10、30及 5 0 μg/ml的脂多糖 (lipopolysaccharide ,LPS) ,10μg/mlLPS + 10 μmol/ml锌原卟啉Ⅸ (Zn protoporphyrin Ⅸ ,ZnPPⅨ )和单纯 10 μmol/mlZnPPⅨ与培养的NRCMs共同孵育 6h ,以及 10 μg/mlLPS与NRCMs共同孵育 9h和 18h。分别观察细胞HO 1mRNA表达、MDA含量、LDH释放量与台盼蓝摄取率的变化。结果显示 ,同样与细胞孵育 6h ,LPS 10 μg/ml时HO 1mRNA表达比对照组增加 81 2 % ,30μg/ml时表达量增加 12 6 3% ,5 0 μg/ml时表达量增加 92 8% ;LPS为 10 μg/ml时 ,孵育 9h后HO 1mRNA的表达量比对照组增加 93 6 % ,孵育 18h后表达量增加 10 5 8%。LPS 30、5 0 μg/ml,10 μg/mlLPS + 10 μmol/mlZnPPⅨ与细胞孵育6h及LPS 10 μg/ml孵育 18h后 ,细胞MDA含量、LDH释放量与台盼蓝摄取率明显增加 (P <0 0 1) ;单纯 10 μg/mlLPS与单纯 10 μmol/mlZnPPⅨ孵育 6h后 ,上述指标均无明显升高。结果表明 ,LPS可诱导NRCMsHO 1mRNA的表达 ,且在较低LPS剂量范围内具有时间依赖性和浓度依赖性 ;NRCMsHO 1mRNA的表达可减低LPS引起的细胞损伤 ,这可
To investigate the expression of hemeoxygenase 1 (HO 1) gene and its role in cell injury in neonatal rat cardiomyocytes (NRCMs) treated with endotoxin, 10, 30 and 5 LPS, 10μg / ml LPS + 10μmol / ml ZnPPⅨ and 10μmol / ml ZnPP were incubated with cultured NRCMs for 6h, and 10μg / ml LPS incubated with NRCMs for 9h and 18h. The changes of HO 1 mRNA expression, MDA content, LDH release and the uptake rate of trypan blue were observed. The results showed that HO 2 mRNA expression increased by 81.2%, 12 6 3% at 30μg / ml and 92 8% at 50 μg / ml, ; When LPS was 10 μg / ml, the expression level of HO 1 mRNA increased 93.6% compared with the control group after 9h incubation, and increased by 105.8% after 18h incubation. LPS 30, 50 μg / ml, 10 μg / ml LPS + 10 μmol / ml ZnPPⅨ for 6 h and LPS 10 μg / ml for 18 h, MDA content, LDH release and trypan blue uptake were significantly increased (P < 0 0 1). No significant increase was observed in the above parameters after incubation with 10 μg / ml LPS and 10 μmol / ml ZnPPⅨ alone for 6 h. The results showed that LPS induced the expression of NRCMsHO1mRNA in a time-dependent and concentration-dependent manner in the lower LPS dose range; the expression of NRCMsHO1mRNA reduced the LPS-induced cell injury,