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以陆地棉品种“珂字２０１”为材料，比较了ＩＡＡ＋ＫＴ和２，４－Ｄ＋ＫＴ在愈伤诱导和悬浮培养中的效应，结果表明，愈伤组织诱导中，ＩＡＡ和２，４－Ｄ表现为正效应，且２，４－Ｄ的效应强于ＩＡＡ；ＫＴ表现为负效应；胚性愈伤悬浮培养中，３种激素都表现出负效应。以胚性细胞悬浮系为材料进行了原生质体的分离和培养试验，分离原生质体的最佳酶组合为纤维素酶（ＯｎｏｚｕｋａＲ１０）３％＋果胶酶（Ｐｅｃｔｉｎａｓｅ）１．５％，原生质体培养的最佳激素组合为ＩＡＡ０．５ｍｇ／Ｌ＋ＫＴ０．１ｍｇ／Ｌ，适当提高原生质体密度有利于原生质体培养。光照，温度等物理因素对原生质体培养也有明显影响。原生质体再生的愈伤组织直接在无激素的悬浮培养基中增殖，很快获得了大量胚性愈伤组织，经分化培养再生出植株
The effects of IAA + KT and 2,4-D + KT on callus induction and suspension culture were compared with the cultivars “Ke-Ci 201” of Upland cotton. The results showed that IAA and 2,4-D showed Positive effect, and 2,4-D effect is stronger than IAA; KT showed negative effect; embryogenic callus suspension culture, all three hormones showed a negative effect. Protoplast isolation and culture experiments were carried out on embryogenic cell suspensions. The optimal enzyme combinations for protoplast isolation were 1.5% of cellulase (Onozuka R10) + 1.5% of pectinase, protoplast culture The optimal hormone combination was IAA0.5mg / L + KT0.1mg / L. Proper protoplast density was favorable for protoplast culture. Light, temperature and other physical factors on protoplast culture also have a significant impact. Callus regenerated from protoplasts propagated directly in hormone-free suspension medium, and a large amount of embryogenic callus was quickly obtained. After differentiation, the plants were regenerated