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目的:酪氨酸羟化酶(tyrosine hydroxylase,TH)是儿茶酚胺类递质合成的限速梅,儿茶酚胺类递质对胰腺内分泌细胞的功能具有重要的调控作用,本研究拟探讨酪氨酸羟化酶(tyrosine hydroxylase,TH)在成年大鼠整个胰腺的具体定位和表达。方法:取雄性成年大鼠胰腺,冰冻组织切片,应用免疫荧光技术观察酪氨酸羟化酶在整个胰腺中的表达分布情况,并进一步运用免疫荧光双标技术鉴定酪氨酸羟化酶是否与胰岛素、胰高血糖素、生长抑素以及胰多肽分别共定位于β细胞;α细胞;δ细胞及PP细胞,进一步确定合成酪氨酸羟化酶确切的细胞类型。结果:①在胰腺腺泡细胞胞浆中存在酪氨酸羟化酶的阳性表达颗粒。②分布于胰腺外分泌腺的神经纤维和胰岛的神经纤维中都有酪氨酸羟化酶的表达。③酪氨酸羟化酶与胰岛的四种内分泌细胞所合成的肽之间均没有共定位关系。结论:在胰腺,酪氨酸羟化酶只存在于胰腺外分泌腺的腺泡细胞胞浆内以及胰腺中的神经纤维中,而胰岛四种内分泌细胞中没有酪氨酸羟化酶,说明胰腺儿茶酚胺类神经递质一方面由胰腺外分泌部的腺泡细胞合成,另一方面来源于神经末梢的释放,而胰岛细胞不能合成儿茶酚胺类递质;该结果为进一步研究胰腺内、外分泌部之间的关系和儿茶酚胺对胰腺分泌功能的调节提供形态学证据。
OBJECTIVE: Tyrosine hydroxylase (TH) is a rate-limiting drug for the synthesis of catecholamines. Catecholamine neurotransmitters play an important regulatory role in the function of pancreatic endocrine cells. This study was designed to investigate the effects of tyrosine hydroxylation The specific localization and expression of tyrosine hydroxylase (TH) in the entire pancreas of adult rats. Methods: The pancreas and frozen tissue sections of male adult rats were used to observe the expression and distribution of tyrosine hydroxylase in the pancreas by immunofluorescence staining. The immunofluorescence double-labeling technique was used to identify whether tyrosine hydroxylase Insulin, glucagon, somatostatin, and pancreatic polypeptide co-localized in beta cells, alpha cells, delta cells, and PP cells, respectively, to further confirm the exact cell type of synthetic tyrosine hydroxylase. Results: ① There was tyrosine hydroxylase positive expression in the cytoplasm of pancreatic acinar cells. ② distributed in the pancreatic exocrine nerve fibers and pancreatic islets have nerve fiber tyrosine hydroxylase expression. ③ tyrosine hydroxylase and islet endocrine cells synthesized by the four peptides are not colocalized relationship. CONCLUSIONS: In the pancreas, tyrosine hydroxylase is found only in the acinar cytoplasm of the pancreatic exocrine gland and in the nerve fibers in the pancreas, whereas there is no tyrosine hydroxylase in the four endocrine cells of the islets indicating that pancreatic catecholamines Neurotransmitters on the one hand by the pancreatic exocrine acinar cells synthesis, on the other hand from the release of nerve endings, and islet cells can not synthesize catecholamines neurotransmitters; the results for further study of pancreatic and exocrine relationship And catecholamines on the regulation of pancreatic secretion provide morphological evidence.