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把MS培养基的各种盐和蔗糖用量减半,辅加BA0.25ppm,IAA0.5ppm及可溶干酪素150ppm做成的培养基,接种近成熟或已成熟山里红种子的种胚,置25～30℃恒温和1000～1500Lux光照的培养室中培养。接种种胚的分化率为84.5％。一枚种胚经10个月的培养、增殖,获得无根绿苗1600多株。把MS培养基的各种成分的用量减半,辅加IBA1.5ppm做成生根培养基,接种3厘米以上的种胚分化苗,生根率为84 .2％。健壮的生根苗,通过砂培过渡3周左右,转至土壤培养,成活率达57.1％。这将为山楂生产快速提供砧木,解决育苗难的问题提出一条有希望的途径。
The medium of MS medium and sucrose dosage in half, supplemented with BA0.25ppm, IAA0.5ppm and soluble casein 150ppm made medium, inoculated nearly mature or ripe mountain red seed embryos, set 25 ~ 30 ℃ constant temperature and 1000 ~ 1500Lux light culture room culture. The rate of differentiation of inoculated embryos was 84.5%. A seed embryo by 10 months of culture, proliferation, access to rootless green seedlings more than 1600 strains. The amount of the various components of MS medium by half, supplemented with IBA1.5ppm made of rooting medium, inoculated 3 cm above embryogenic differentiation seedlings, rooting rate of 84.2%. Sturdy rooting seedlings, through sand culture transition about 3 weeks, go to soil culture, survival rate of 57.1%. This will provide a quick way to provide rootstocks for hawthorn production and provide a promising way to solve the difficult problems of nursery.