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采用超排卵技术使ICR雌性小鼠排卵,排卵前用环磷酰胺(CYC 25、50、100mg/kg)处理后与雄性小鼠交配,分析小鼠卵母细胞(MⅡ)及第一次分裂合子的染色体非整倍体及结构畸变。 对照小鼠MⅡ及第一次分裂合子均未发现有染色体非整倍体及结构畸变。环磷酰胺100mg/kg处理组小鼠MⅡ非整倍体率显著增加,但未见染色体结构畸变。 在小鼠第一次分裂合子中,环磷酰胺可使雌原核染色体非整倍体率增加,并诱发多种染色体结构畸变,畸变率随环磷酰胺剂量的增加而增加。该分析技术具有自然畸变率低、灵敏、快速等 优点。
Ovulation was induced in female ICR mice by superovulation technique. Male mice were mated with cyclophosphamide (CYC 25, 50, and 100 mg / kg) before ovulation. Mouse oocytes (MⅡ) and first mitotic zygotes Aneuploidy and structural aberrations. No chromosomal aneuploidy and structural aberrations were found in the control mice MII and the first mitotic. Cyclophosphamide 100mg / kg treatment group MII aneuploidy rate was significantly increased, but no chromosomal structural aberrations. Cyclophosphamide can increase the rate of aneuploidy in progesterone chromosomes and induce a variety of chromosomal structural aberrations in the first division of zygotes in mice. The rate of aberrations increases with the dose of cyclophosphamide. The analysis technology has the advantages of low natural distortion rate, sensitivity, fastness and so on.