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为了阐明常温下缺血心肌组织pH的变化与心肌细胞内pH和生物能之间的关系,作者选择体重20～22kg的健康犬14条,全麻后进胸显露心脏,持续监测心肌温度,MAP、LVEDP、HR、dp/dt max、心肌组织pH(壁内电极法)、心肌细胞pH(31~p-NMR法)及ATP并记录.然后将犬分成2组,组Ⅰ(n=7)为实验组,分离并阻断左冠状动脉前降支(LAD),使该动脉灌注区完全缺血,再重复监测上述参数,持续3hr.组Ⅱ(n=7)为对照组,除不阻断LAD外,余均同组Ⅰ.结果:两组犬的心肌温度均保持在36℃.两组血流动力学参数(MAP、dp/dt max、LVEDP等)在实验期间均无明显变化,组Ⅰ的心肌组织pH值自LAD阻断前的7.38±0.02降至阻断3hr后的5.90±0.13,与组
In order to clarify the relationship between the change of pH in ischemic myocardium and the intracellular pH and bioenergetic energy at normal temperature, we selected 14 healthy dogs weighing 20 ~ 22kg and exposed the heart to the chest after general anesthesia. The myocardial temperature, MAP, LVEDP, HR, dp / dt max, myocardial tissue pH (intramural electrode method), cardiomyocyte pH (31 ~ p-NMR) and ATP were recorded and divided into two groups. In the experimental group, the left anterior descending artery (LAD) was isolated and blocked, and the artery perfusion area was completely ischemia, and the above parameters were repeated for 3 hours. Group II (n = 7) LAD, the results were the same as in group I. Results: Myocardial temperature kept at 36 ℃ in both groups.The hemodynamics parameters (MAP, dp / dt max, LVEDP, etc.) in both groups had no significant change during the experiment, The myocardial pH of Ⅰ decreased from 7.38 ± 0.02 before LAD to 5.90 ± 0.13 after 3hr,