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目的 探讨核因子 κB(Nuclearfactor κB ,NF κB)在哮喘患儿气道炎症中的作用。方法 获得 9例哮喘患儿及 6例非哮喘对照的支气管粘膜 ,进行免疫组化及凝胶电泳迁移率检查 ,分别观察NF κB在支气管上皮细胞的表达及NF κB与DNA的结合活性。结果 NF κB在 9例哮喘患儿的支气管上皮细胞核均有表达 ;在 4例哮喘患儿中 (对 6例哮喘患儿进行了凝胶电泳迁移率检查 ) ,观察到NF κB与DNA结合。在 6例非哮喘对照患儿的支气管上皮细胞核均未见NF κB表达 ,也未见NF κB与DNA结合。结论 NF κB在哮喘患儿支气管上皮细胞活化 ,可能通过调控多种炎性蛋白的表达 ,导致气道炎症发生。
Objective To investigate the role of nuclear factor κB (NF κB) in airway inflammation in children with asthma. Methods Bronchial mucosa of 9 asthmatic children and 6 non-asthmatic controls were obtained. The expression of NF κB in bronchial epithelial cells and the binding activity of NF κB to DNA were detected by immunohistochemistry and gel electrophoresis. Results NFκB was expressed in the nuclei of bronchial epithelial cells in all 9 asthmatic children. In 4 asthmatic children (6 cases of asthmatic children were examined by gel electrophoresis), NF κB was observed to bind with DNA. No NF-κB expression was found in the bronchial epithelial nuclei of 6 non-asthmatic children, nor was NF κB bound to DNA. Conclusion NF κB activation in bronchial epithelial cells in asthmatic children may lead to airway inflammation by regulating the expression of many inflammatory proteins.