研究并比较了 4种金属离子 Hg( )、Mn( )、Zn( )、Cd( )在碱性条件下催化 β-内酰胺类抗生素水解的能力 ,发现 Hg( )、Zn( )对 β-内酰胺类抗生素水解的催化作用效果良好 ,而 Mn( )和 Cd( )对长效青霉素水解的催化作用效果不明显。提出了分别在 Hg( )和 Zn( )催化作用下测定β-内酰胺类抗生素的荧光光谱分析法 ,并用于体液中抗生素残留物的检测。 The ability of four kinds of metal ions Hg (), Mn (), Zn () and Cd () to catalyze the hydrolysis of β-lactam antibiotics under alkaline conditions was studied and compared. The catalytic effect of lactam antibiotic hydrolysis was good, while the effect of Mn () and Cd () on the hydrolysis of long-acting penicillin was not obvious. Fluorescence spectrometry for the determination of β-lactam antibiotics under the catalysis of Hg () and Zn () has been proposed and used to detect the residues of antibiotics in body fluids.