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利用10对多态性丰富的SSR引物,以国家甘蔗种质资源圃中保存的14份具有代表性的割手密为对照,对新收集的云南省11份割手密(Saccharum spontaneum L.)资源进行多样性分析。结果共扩增出233条DNA谱带,与对照相比,新采集材料的多态性条带为207条,其中14条为特有条带,多态性条带比率为0.89。遗传相似性系数和UPGMA聚类分析表明,新采集的材料并没有单独聚为一类,而是比较分散,在相似性系数为0.64处作切割线,参试材料可分为3个类群:第Ⅰ类群主要由龙门割手密、河边村割手密和福建仙游1号组成;第Ⅱ类群中包含19份材料,其中新采集的样品有上岗割手密、他拉割手密、安乐割手密、勐根割手密、芒美割手密、贺海割手密、回落割手密、里拉割手密和曼亨割手密,对照材料主要包含了云南、四川、越南、老挝、泰国地区的割手密,其共同特点是均匀分布在内陆地区;第Ⅲ类群包括3个材料,分别是海南1号、海南92-2和广东化州割手密,其中不包含新采集的材料。而在相似性系数为0.654处作切割线又能将上述第Ⅱ类群分为较细的3个亚群。由此可见,新采集的11份割手密资源具有丰富的遗传多样性,与已收集的资源相比,具有一定的差异性。说明依靠云南高山峡谷等立体气候特点,分布着遗传差异显著的割手密无性系。
Ten pairs of SSR primers rich in polymorphism were used as controls to compare 14 newly collected Saccharum spontaneum L. collected from Yunnan Province with 14 representative cuttings secreted from the national sugarcane germplasm resource garden. Resources for diversity analysis. A total of 233 DNA bands were amplified. Compared with the control, the number of polymorphic bands of the newly collected materials was 207, of which 14 were endemic bands and the polymorphic bands were 0.89. Genetic similarity coefficient and UPGMA clustering analysis showed that the newly collected materials were not grouped together, but rather dispersed, with the similarity coefficient of 0.64 as the cutting line. The tested materials could be divided into three groups: Group Ⅰ is mainly composed of gantry cut-off, cuttings of Haipian village and No. 1 of Xianyou, Fujian province. The second group contains 19 materials, among which the newly collected samples have dense cuttings, Cut the hand dense, Meng root cut the secret, Man-Mi cut the hand-dense, Hehai cut the hand dense, fall cut the hand cut, Lila cut the hand close and Manchu cut the secret, the control material mainly contains Yunnan, Sichuan, Vietnam, Laos , Thailand, cutting the secret, its common feature is evenly distributed inland; Group Ⅲ includes three materials, respectively, Hainan 1, Hainan 92-2 and Guangdong Huazhou cut the secret, which does not include the new acquisition s material. However, when the similarity coefficient is 0.654, it can divide the above-mentioned group II into three sub-groups. This shows that the 11 newly collected resources have a wealth of genetic diversity, compared with the resources collected, with some differences. It shows that the clonal clones with significant genetic differences are distributed on the basis of three-dimensional climatic characteristics, such as the alpine gorges in Yunnan.