Resistance to maize southern leaf spot disease was induced by the low-concentration filtrate of Bipolaris maydis race T cultivation in an experiment. The nuclear neterogeny corn C103 was used as the test material. The lesion area on the leaves was significant difference by connalysis. The lesion areas on pretreated leaves were (0.3 ± 0.05)- (0.9 ± 0.5) mm2, but those on the control were (23.1 ± 8.7) mm2. At the same time, the changes in peroxidase, phenylalanine, ammonialyase, and malondialdehvde activities were determined. During 0-96 h of inspection, phenylalanine and ammonialyase (PAL) activities increased by 64.2%, peroxidase (POD) activities increased by 41.2%, but the malondialdenvde (MDA) content decreased by 29.7% compared with the control. It seems that the low-concentration filtrate of Bipolaris maydis race T cultivation itself can be used as an elicitor to enhance the induced resistance.