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目的探讨用油基法蓖麻油试验鉴定马拉色菌。方法制作含不同浓度蓖麻油的沙堡弱液体培养基(SDB),将糠秕马拉色菌菌悬液加入其中,37℃振荡培养。在第4、6、8、101、2 d分别对各培养液中生长的马拉色菌孢子数计数,确定适于糠秕马拉色菌生长的蓖麻油最佳浓度。将11种马拉色菌标准株接种在含此浓度蓖麻油的沙堡弱培养基琼脂(SDA)上,观察菌落的生长情况。以传统方法作11种马拉色菌标准株的蓖麻油试验。观察比较两种试验方法及结果。结果糠秕马拉色菌在含4%蓖麻油的SDB中生长最多(P<0.05)。在含4%蓖麻油SDA上,11种马拉色菌标准株生长情况与传统方法实验结果一致。油基法操作简便且所需试剂及菌量明显少于传统方法。结论油基法蓖麻油试验实验结果与传统方法一致,可提高鉴定马拉色菌的效率和经济性。
Objective To investigate the identification of Malassezia by oil-based castor oil test. Methods Sandcastle weak liquid medium (SDB) with different concentrations of castor oil was prepared, and the suspension of Malassezia furfur was added to shake culture at 37 ℃. On the 4th, 6th, 8th, the 10th, the 2nd and the 2nd day, the number of Malassezia spores grown in each culture medium was counted to determine the optimal castor oil concentration for the growth of Malassezia furfur. Eleven species of Malassezia were inoculated on the sandcastle weak medium agar (SDA) containing this concentration of castor oil to observe the growth of the colonies. Castor oil test of 11 standard strains of Malassezia by traditional method. Observation and comparison of two test methods and results. Results Malassezia furfur grew most in SDB with 4% castor oil (P <0.05). The growth of 11 standard strains of Malassezia on 4% castor oil SDA was consistent with that of the traditional method. Oil-based method is simple and requires less reagents and bacteria than traditional methods. Conclusion The experimental results of the oil-based castor oil test are consistent with the traditional methods, which can improve the efficiency and economy of the identification of Malassezia.