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目的通过检测CDK4、ki67和PTEN在子宫内膜癌组织中表达情况来探讨其意义。方法应用免疫组织化学染色S-P法检测60例子宫内膜癌、32例正常子宫内膜组织中三者的表达情况,计算阳性率并进行分析。结果子宫内膜癌组织中CDK4和ki67表达定位于细胞核,其阳性表达率分别为78.33%(47/60)、85.00%(51/60),明显高于正常子宫内膜组织25.00%(8/32)和12.50%(4/32),差异有统计学意义(P<0.05)。PTEN蛋白在子宫内膜癌组织中缺失率为73.33%,高于正常子宫内膜组织(P<0.05)。CDK4和ki67在不同组织学分级、不同临床病理分期以及是否有淋巴结转移的子宫内膜癌中的表达,差异均有统计学意义,(P均<0.05);而在不同组织学亚型及不同肌层浸润程度中的表达,差异无统计学意义(P均>0.05)。PTEN蛋白表达在肌层浸润深度、组织学亚型、淋巴结转移及手术-病理分期中差异无统计学意义(P>0.05),而且PTEN表达在G1级肿瘤高于G2、G3级(P<0.05),不同肿瘤组织学亚型无关,PTEN蛋白缺失率差异无统计学意义(P>0.05),在子宫内膜癌组织中CDK4和ki67两者表达呈正相关(P<0.05),CDK4与PTEN的表达无明显相关性。结论 CDK4和ki67在子宫内膜癌呈高表达和PTEN蛋白表达缺失,且与子宫内膜癌的发生、发展和转移具有一定的关系。
Objective To explore the significance of CDK4, ki67 and PTEN expression in endometrial carcinoma. Methods The expression of three of 60 cases of endometrial carcinoma and 32 cases of normal endometrium were detected by immunohistochemical S-P method. The positive rate was calculated and analyzed. Results The positive expression rates of CDK4 and ki67 in endometrial carcinoma were 78.33% (47/60) and 85.00% (51/60), respectively, which were significantly higher than those in normal endometrium (25.00%, 8 / 32) and 12.50% (4/32) respectively, the difference was statistically significant (P <0.05). The loss rate of PTEN protein in endometrial carcinoma was 73.33%, higher than that in normal endometrium (P <0.05). CDK4 and ki67 in different histological grade, different clinical pathological stages and whether there is lymph node metastasis of endometrial cancer, the differences were statistically significant (P all <0.05); in different histological subtypes and different The expression of myometrial invasion, the difference was not statistically significant (P all> 0.05). There was no significant difference in the expression of PTEN protein in the depth of myometrial invasion, histological subtypes, lymph node metastasis and surgical-pathological staging (P> 0.05) ). There was no significant difference in the deletion rate of PTEN between different tumor histological subtypes (P> 0.05). The expression of CDK4 and ki67 in endometrial carcinoma was positively correlated (P <0.05) No significant correlation between expression. Conclusions CDK4 and ki67 are highly expressed in endometrial carcinoma and lack of PTEN protein expression, and have a relationship with the occurrence, development and metastasis of endometrial carcinoma.