水稻离体穗杂交,父本花粉采集难是常碰到的问题,如不开花,或花粉量极少,尤以粳稻父本穗的情况为甚。我们采用了下述方法保证了父本花粉的及时和充分供应。上午八时前,抽取父本穗(不带叶)立即插入装水的试管中 In vitro hybridization of rice ears, pollen collection is difficult to encounter the parent is often the problem, such as not flowering, or very little pollen, especially in the case of male parent panicle japonica. We have adopted the following method to ensure the timely and adequate supply of male pollen. Before 8:00 am, the male parent’s spike (without leaves) was immediately inserted into a water-filled test tube