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[目的]研究金钗石斛多糖对脂多糖(LPS)诱导的小鼠腹腔巨噬细胞分泌肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)的影响。[方法]用不同浓度金钗石斛多糖作用于小鼠腹腔巨噬细胞,LPS作为诱导剂,采用放射免疫法检测细胞培养液中TNF-α的含量,硝酸酶还原法测定细胞培养液中NO的含量,荧光法测定细胞内诱导型一氧化氮合酶(iNOS)的活性,实时PCR(real-time PCR)法测定TNF-αmR-NA、iNOS mRNA的表达。[结果]与LPS组比较,金钗石斛多糖在50～400mg/L范围内可干预LPS对小鼠巨噬细胞的作用,使小鼠腹腔巨噬细胞TNF-α、NO合成减少,iNOS活性降低,TNF-αmRNA、iNOS mRNA的表达降低。[结论]金钗石斛多糖可改善LPS对小鼠腹腔巨噬细胞的作用,使TNF-αmRNA、iNOS mRNA的表达降低,TNF-α、NO合成减少,从而起到抗炎的作用。
[Objective] To investigate the effects of polysaccharides from Dendrobium nobile on secretion of tumor necrosis factor-α (TNF-α) and nitric oxide (NO) induced by lipopolysaccharide (LPS) in mouse peritoneal macrophages. [Method] With different concentration of polysaccharides of Dendrobium nobile Lindl. In mice peritoneal macrophages, LPS as an inducer, radioimmunoassay was used to detect the content of TNF-α in the cell culture medium, nitrite reduction method was used to determine the content of NO The activity of inducible nitric oxide synthase (iNOS) was measured by fluorescence method. The expression of TNF-αmR-NA and iNOS mRNA was detected by real-time PCR. [Result] Compared with LPS group, the polysaccharide from Dendrobium nobile could intervene LPS on mouse macrophages in the range of 50 ~ 400 mg / L, and decrease the synthesis of TNF-α, NO and the activity of iNOS in mouse peritoneal macrophages , TNF-α mRNA, iNOS mRNA expression decreased. [Conclusion] Dendrobium nobile polysaccharide could improve the effect of LPS on mouse peritoneal macrophages, decrease the expression of TNF-α mRNA and iNOS mRNA, decrease the synthesis of TNF-α and NO, and thus play an anti-inflammatory role.