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用改进的全电化学三步法制备三维金纳米团簇/多壁碳纳米管(3D Au/MWCNTs)纳米复合材料,并用Nafion(Nafion)膜进行涂布固定,制得3D Au/MWCNTs-Nafion修饰电极.利用透射电子显微镜(TEM)和能量色散光谱(EDS)对所得纳米复合材料的形貌进行表征.3D Au/MWCNTs具有金纳米核团簇而成的特殊圆丘状三维结构,电化学活性表面积(ECSA)比均匀分散的Au/MWCNTs提高了一个数量级,可有效提高血红蛋白(Hb)在电极表面的负载量.运用循环伏安法和计时电流法对3D Au/MWCNTs-Nafion修饰电极的生物电催化性质进行研究,其在Hb溶液中显示了良好的电催化活性和稳定性:还原氧化峰电流高,反应可逆性好,提供了有利于Hb直接电子转移的电化学环境.固载于Au/MWCNTs-Nafion上的Hb能够保持其生物活性,对双氧水(H2O2)表现出良好的催化性能,这是3D Au纳米团簇和MWCNTs共同作用的结果.实验表明,3D Au/MWCNTs-Nafion修饰电极结构特殊、性能优越,对Hb的直接电化学研究具有积极的促进作用,为准确高效的检测Hb及相关生物活性物质提供了新的电极选择.
Three-dimensional Au / MWCNTs nanocomposites were prepared by a modified all-electrochemical three-step method and coated with Nafion (Nafion) membrane. 3D Au / MWCNTs-Nafion The morphology of the nanocomposites was characterized by transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) .3D Au / MWCNTs have a special dome-shaped three-dimensional structure with AuNuclear clusters, and the electrochemical The active surface area (ECSA) increased by one order of magnitude compared with that of the uniformly dispersed Au / MWCNTs, which could effectively increase the hemoglobin (Hb) loading on the electrode surface.Using cyclic voltammetry and chronoamperometry for 3D Au / MWCNTs-Nafion modified electrode Bioelectrocatalytic properties of the Hb solution showed good electrocatalytic activity and stability in Hb solution. High reduction-oxidation peak current and good reversibility of the reaction provided an electrochemical environment conducive to the direct electron transfer of Hb. Hb on Au / MWCNTs-Nafion can maintain its biological activity and exhibit good catalytic activity on hydrogen peroxide (H2O2), which is the result of the interaction between 3D Au nanoclusters and MWCNTs. The experiment shows that 3D Au / MWCNTs-Nafion modification Electrode junction Special, superior performance, the direct electrochemical studies of Hb has a positive role in promoting, providing new options for accurate and efficient electrode for detecting Hb and related biologically active substances.